Detection and typing of human papillomaviruses by in situ hybridization with biotinylated oligonucleotide mixtures
Identifieur interne : 000337 ( France/Analysis ); précédent : 000336; suivant : 000338Detection and typing of human papillomaviruses by in situ hybridization with biotinylated oligonucleotide mixtures
Auteurs : Marie-Lise Jourdan [France] ; Alain Goudeau [France] ; Martine Joannes [France] ; C Me Barranger [France] ; Gérard Sommé [France]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 1995-03.
Abstract
The value of biotinylated Oligonucleotide probes for screening and typing by in situ hybridization of the most frequent genital human papillomavirus infections (HPVs 6,11,16,18, 31, and 33) was assessed. Optimal hybridization conditions were defined on a panel of paraffin‐embedded tissue sections previously characterized with HPV full genome probes. Mixtures of oligonucleotides rather than single oligonucleotides were used to improve sensitivity and specificity. All HPV‐positive specimens were detected by the screening mixture with a sensitivity and specificity similar to that of full genome probes. Typing mixtures were highly specific for each HPV type. This study confirms the potential of Oligonucleotide probes for detecting and typing HPV infections. © 1995 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.1890450310
Affiliations:
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type="city">Paris</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">45</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="293">293</biblScope><biblScope unit="page" to="299">299</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1995-03">1995-03</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The value of biotinylated Oligonucleotide probes for screening and typing by in situ hybridization of the most frequent genital human papillomavirus infections (HPVs 6,11,16,18, 31, and 33) was assessed. Optimal hybridization conditions were defined on a panel of paraffin‐embedded tissue sections previously characterized with HPV full genome probes. Mixtures of oligonucleotides rather than single oligonucleotides were used to improve sensitivity and specificity. All HPV‐positive specimens were detected by the screening mixture with a sensitivity and specificity similar to that of full genome probes. Typing mixtures were highly specific for each HPV type. This study confirms the potential of Oligonucleotide probes for detecting and typing HPV infections. © 1995 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Centre-Val de Loire</li><li>Région Centre</li><li>Île-de-France</li></region><settlement><li>Paris</li><li>Tours</li></settlement></list><tree><country name="France"><region name="Centre-Val de Loire"><name sortKey="Jourdan, Marie Ise" sort="Jourdan, Marie Ise" uniqKey="Jourdan M" first="Marie-Lise" last="Jourdan">Marie-Lise Jourdan</name></region><name sortKey="Barranger, C Me" sort="Barranger, C Me" uniqKey="Barranger C" first="C Me" last="Barranger">C Me Barranger</name><name sortKey="Goudeau, Alain" sort="Goudeau, Alain" uniqKey="Goudeau A" first="Alain" last="Goudeau">Alain Goudeau</name><name sortKey="Goudeau, Alain" sort="Goudeau, Alain" uniqKey="Goudeau A" first="Alain" last="Goudeau">Alain Goudeau</name><name sortKey="Joannes, Martine" sort="Joannes, Martine" uniqKey="Joannes M" first="Martine" last="Joannes">Martine Joannes</name><name sortKey="Somme, Gerard" sort="Somme, Gerard" uniqKey="Somme G" first="Gérard" last="Sommé">Gérard Sommé</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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